In-vitro study of house dust mite allergy

Dra. Med. Linda Rahadian

Background

House dust mite is a source of allergens that can cause allergies’. From the biophysical aspect, the occurrence of allergies in the human body is the result of the development of allergy imprinting which comes from human body contact with a substance; printing in the form of information of the substance. Repeated printing will make biophysical pulses that produce symptoms. Each allergen has traces of bioresonance patterns only from the appropriate allergens.2

The study is conducted with an experimental design to assess the impact of bioreso­nance of the electromagnetic waves on the changes in pro-inflammatory mediator pro­files (Interleukin 4 and 13) and anti-inflammatory mediator (Interleukin 10) which are produced by complete blood cell cultures drawn from subjects of Rhinitis Allergy caused by house dust mite (Type-1 Hypersensitivity).

Several clinical studies, such as Song Ki Min and Du Xia et al had shown improvement in clinical symptoms for allergy sufferers who were treated with bioresonance.3,4 What is actually happening at the cellular level for bioresonance to produce symptomatic im­provement? It is not yet known whether it is due to changes in some immunological markers of type-1 hypersensitivity.

From the aspect of immunology, type-1 hypersensitivity as the immunological disor­der, is observed from the increased activity of cellular immunity such as of type-2 pro-inflammatory mediators Interleukin 4 (IL4) and Interleukin 13 (IL13). Both levels are in­creased .5

One of the factors of increased level of type-2 pro-inflammatory mediator is caused by decreased function of anti-inflammatory mediator such as Interleukin 10 (IL10).5 Thus, it is interesting to know whether bioresonance treatment in the blood cell cultures can affect mediator profiles of type-2 pro-inflammatory mediators such as IL4, L13 and

anti- inflammatory mediator IL10.

Interleukin 4 and 13 (IL4 & IL13):

Type 2 pro-inflammation mediators, produced by immune cells such as Thelper2 (Th2). These cytokines play an important role in the modulation of type 1 hypersensitivity.5

Interleukin 10 (IL10):

Anti-inflammation mediator, produced mainly by immune cells and majorly by Lym­phocyte T regulator (Treg). This cytokine plays an important role as a tolerance media­tor, which has antagonic effect against type 1 or 2 Pro-inflammation mediator/s.5

Methodology

The study is conducted with an in vitro experimental design. Blood sampling is done on the respondents with positive anti mite Immunoglobulin E (IgE) level as the inclusive criteria. The sample is taken from 16 subjects, divided into 2 groups: A and B with 8 subjects each. The A group is made double, Al and A2.

Al is a group of blood cultures treated with bioresonance. B is a group of blood cul­tures without bioresonance treatment. The A2 samples are the same with Al, but A2 is not treated by bioresonance. There are negative control, positive control and tube of mite stimulation for each subject, for day 1, 5 and 7 (H1, H5 and H7).

Levels of IL 4 and IL13 and IL 10 are measured with Elisa technique. The device for bioresonance treatment is BICOM BICOM optima® B22

Procedure

Day 1 (H1):       BICOM Bioresonance treatment only on Al group.

Program 963. Duration depends on each blood culture Day 2 (H2):   BICOM Bioresonance treatment only on Al group.

Program 944. Duration depends on each blood culture Day 3 (H3):   BICOM Bioresonance treatment only on Al group.

Program 998. Duration depends on each blood culture

Day 4 (H4):       Stimulation of house dust mite allergen on all groups

Day 5 (H5):       Collection on supernatant on all groups

Day 7 (H7):       Collection on supernatant on all groups

Research Purposes:

1. To analyze the effect of Bioresonance towards the change of IL4, IL13, and IL10

2. To analyze the level of IL4, IL13, and 110 before and after the treatment of Biore­sonance

3. To analyze the correlation between the pro-inflammation and the anti-inflamma­tion mediators on house dust mite-stimulated blood cultures

Research Result

1. Bioresonance can change the production pattern of type 2 pro-inflammatory medi­ators (IL4 and IL13) as a specific response to the stimulation of mite allergens.

2. Post treatment of Bioresonance in blood cultures causes immune cells in those cul­tures significantly lower in producing type-2 inflammatory mediators (IL4 and IL13) when stimulated by mite allergens. And conversely it increases IL10 anti-inflamma­tory mediator production.

3. The analysis shows that type-2 pro-inflammatory cytokines (IL4 and IL13) with 1110 anti-inflammatory cytokines produced by immune cells from complete blood cul­tures stimulated by house dust mite allergens (mite) and those given bioresonance treatment turned out to be negatively correlated (antagonic action). In the group with bioresonance treatment, the higher anti-inflammatory production of IL10 from immune cells of the complete blood cultures stimulated by mite allergens caused the production of type 2 pro-inflammatory cytokines both IL4 and 1113 sig­nificantly decreased.

Conclusions

1. Bioresonance creates response to immune cells and that is assessed by changes in cytokine production both IL4, IL13 and IL10 in complete blood cell cultures.

2. Bioresonance decreases the production of type-2 inflammatory mediators (IL4 and IL13) produced by immune cells in house dust mite stimulated blood cell cultures.

3. Bioresonance increases the production of type-2 anti-inflammatory mediator (IL10) produced by immune cells of house dust mite stimulated blood cell cultures.

4. Bioresonance does not change the biological function of anti-inflammation role which inhibits the production of the pro-inflammation mediators (antagonic func­tion)

5. Bioresonance can induce and resonate specifically to the same immune cells from the same subject (cells communicate)

Closing

Based on the result, this research is significantly useful as the first step for further ex­periments with BICOM Bioresonance to explore deeper on the cellular level, especially on the effect of Tregulator which its anti-inflammatory function, not only for allergies but for other diseases such autoimmune and cancer.

References

1. Calderon MA, Linneberg A, Kleine-Tebbe J, et al. Respiratory allergy caused by house dust mites: What do we really know? Journal Allergy and Clinical 2015; 136 (1): 38-48.

2. Hennecke J. Allergy and osccilation. Germany: Maller-Schafus; 2003

3. Song Ke-min, Yang Rong-ya, Ao Jun-hong, Zhang Jie. The clinical study of BICOM Journal of Clinical Dermatology 2003-10

4. Du Xia et al. Clinical Observation Of 79 cases of allergic skin disease treated with the bioresonance Chinese Journal of Practical Medicine. 2005; 4(3).

5. Abbas AK, Lichman AH. Cellular and molecular immunology, Eight edition. Philadel­phia: Elsevier Daunders; 2005:417-422 p

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